1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine: a novel source of arachidonic acid in neutrophils stimulated by the calcium ionophore A23187

Biochem Biophys Res Commun. 1983 May 31;113(1):72-9. doi: 10.1016/0006-291x(83)90433-3.

Abstract

Rabbit peritoneal neutrophils incorporated [14C]arachidonic acid into seven molecular species of choline-containing phosphoglycerides. These 2-[14C]arachidonoyl species differed with respect to the alkyl ether or acyl residue bound at the sn-1 position; four of the seven were ether-linked. Stimulation with calcium ionophore A23187 induced a proportionate release of arachidonate from all seven molecular species: 40% of the released arachidonate came from alkyl ether species. Thus, 1-O-alkyl-2-arachidonoyl-sn-glycero-3-phosphocholine (GPC) is a significant source of metabolizable arachidonic acid. Since 1-O-alkyl-2-lyso-GPC is the metabolic precursor of platelet activating factor, these results further interrelate pathways forming arachidonate metabolites and platelet activating factor; they also supply a rationale for the observation that both classes of stimuli form concomitantly during cell activation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Arachidonic Acid
  • Arachidonic Acids / blood*
  • Calcimycin / pharmacology*
  • Chromatography, High Pressure Liquid
  • Neutrophils / drug effects
  • Neutrophils / metabolism*
  • Platelet Activating Factor / blood*
  • Rabbits
  • Stearic Acids / blood

Substances

  • Anti-Bacterial Agents
  • Arachidonic Acids
  • Platelet Activating Factor
  • Stearic Acids
  • Arachidonic Acid
  • Calcimycin
  • stearic acid