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Biomed Biochim Acta. 1983;42(4):343-9.

A rapid procedure for the preparation of highly purified pyruvate decarboxylase from brewer's yeast.


A rapid purification procedure for pyruvate decarboxylase (E.C. from fresh cells of brewer's yeast (Saccharomyces carlsb.) is reported. The preparation of a crude enzyme (30-45 U/mg) by the use of fractionation steps with protamine sulfate, acetone, and ammonium sulfate takes about 6-7 h. A stable pyruvate decarboxylase (70-85 U/mg) was obtained from such preparations after purification on CM Sephadex C 50 after another 2-3 h. Stability and structural properties are compared for enzymes prepared from fresh and dried yeast.

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