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Cell. 1983 Jun;33(2):629-37.

RecA-independent recombination between direct repeats of IS50.

Abstract

Recombination between the IS50 sequences that flank Tn5 has been found to occur readily after the transformation of E. coli recA strains with a plasmid that contains direct repeats of these sequences. Analysis of mutants of IS50 indicates that the polypeptide encoded by IS50 that is required for transposition is also required for the recombination. Surprisingly, the mechanism of recombination appears to be similar to general homologous recombination rather than site-specific recombination. This IS50 recombination system may be responsible for resolving transient cointegrate structures containing direct repeats of IS50 or Tn5 during the transposition of IS50 and Tn5.

PMID:
6345001
DOI:
10.1016/0092-8674(83)90444-0
[Indexed for MEDLINE]

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