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Plasmid. 1983 Mar;9(2):182-90.

Determination of plasmid copy number by fluorescence densitometry.

Abstract

A simple and reliable method for the determination of plasmid copy numbers by direct fluorescence densitometry of ethidium bromide-stained electrophoretic gels was developed. In developing the method, the following parameters were evaluated and controlled: plasmid DNA trapping in the linear chromosomal DNA, staining-destaining kinetics for ethidium bromide, linearity of the fluorescence response, and the effect of the molecular topology of DNA on ethidium bromide binding to DNA in agarose.

PMID:
6344110
[Indexed for MEDLINE]

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