Format

Send to

Choose Destination
Nucleic Acids Res. 1984 Jan 25;12(2):887-99.

The nucleotide recognized by the Escherichia coli A restriction and modification enzyme.

Abstract

The nucleotide recognition sequence for the restriction-modification enzyme of Escherichia coli A (EcoA) has been determined to be GAG-7N-GTCA. This sequence is fairly similar, but distinctly different from the two other type I restriction enzyme recognition sites known for E. coli B and E. coli K12, respectively. N6-adenosine methylation has been observed at nucleotide positions 2 and 12 within that sequence after modification by EcoA. As a reference point for mapping the single EcoA site in lambda, the position of lambda point mutation Oam29 has been determined also.

PMID:
6320124
PMCID:
PMC318543
DOI:
10.1093/nar/12.2.887
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center