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Gene. 1983 Sep;24(1):125-9.

An IS15 insertion generates an eight-base-pair duplication of the target DNA.

Abstract

In plasmid pIP1088 the transposable module IS15 is inserted at nucleotide position 1,430 of the vector plasmid pBR322. We have sequenced the termini of the IS15 element, which consists of two perfect inverted repeat sequences, 14 bp long. The sequence is 5'-GGCACTGTTGCAAA . . . TTTGCAACAGTGCC-3'. The integration event results in the duplication of 8 bp of target DNA.

PMID:
6313479
DOI:
10.1016/0378-1119(83)90137-3
[Indexed for MEDLINE]

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