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Nucleic Acids Res. 1983 Sep 10;11(17):5855-64.

Essential structure of E. coli promoter: effect of spacer length between the two consensus sequences on promoter function.

Abstract

A promoter with the consensus sequence(TTGACA and TATAAT) at -35 and -10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 base-pairs regardless of the sites of space adjustment. Evidence was also provided that the start point of transcription was fixed by the consensus sequence at the -10 region.

PMID:
6310517
PMCID:
PMC326322
DOI:
10.1093/nar/11.17.5855
[Indexed for MEDLINE]
Free PMC Article

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