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Methods Enzymol. 1983;101:202-11.

One-step gene disruption in yeast.

Abstract

The one-step gene disruption techniques described here are versatile in that a disruption can be made simply by the appropriate cloning experiment. The resultant chromosomal insertion is nonreverting and contains a genetically linked marker. Detailed knowledge of the restriction map of a fragment is not necessary. It is even possible to "probe" a fragment that is unmapped for genetic functions by constructing a series of insertions and testing each one for its phenotype.

PMID:
6310324
DOI:
10.1016/0076-6879(83)01015-0
[Indexed for MEDLINE]

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