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Virology. 1983 Jul 15;128(1):92-104.

Pneumococcal bacteriophage Cp-1 contains a protein bound to the 5' termini of its DNA.

Abstract

The genome of the pneumococcal bacteriophage Cp-1 has been isolated as a DNA-protein complex. The transfecting activity of this complex is destroyed by treatment with proteolytic enzymes. The DNA-protein complexes do not enter into agarose or acrylamide gels and are retained on glass fiber filters. The protein is specifically associated with the two 5' termini of Cp-1 DNA on the basis of experiments carried out with restriction endonucleases, exonucleases, and radioactive labeling with [gamma-32P]ATP and polynucleotide kinase. The protein component, iodinated in vitro with 125I, has a molecular weight of 28,000 determined by SDS-polyacrylamide gel electrophoresis. The protein remains associated with the Cp-1 DNA after thermal or alkali denaturation, incubation with 6 M guanidinium chloride or 8 M urea, and boiling in 2% SDS, 2% mercaptoethanol, and 6 M urea. When the complex was incubated in 1 M sodium hydroxide or 2.5 M piperidine only a partial breakage of the DNA-protein bond was observed. These results indicate that the 28,000-Da protein is covalently bound to the 5' termini of the DNA.

PMID:
6308899
DOI:
10.1016/0042-6822(83)90321-5
[Indexed for MEDLINE]

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