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Br J Cancer. 1983 May;47(5):681-6.

Characterisation of Epstein-Barr virus-specific memory T cells from the peripheral blood of seropositive individuals.


We have investigated the regression phenomenon which occurs when EBV-infected peripheral blood mononuclear cells from seropositive individuals are cultured for one month at high cell concentration and have confirmed that regression is mediated by E+ lymphocytes. When helper/inducer (Leu 3a+) and suppressor/cytotoxic (Leu 2a+) cells are separated by fluorescence-activated cell sorting from fresh peripheral blood and co-cultured with EBV-infected autologous E- mononuclear cells, regression only regularly occurs in cultures receiving suppressor/cytotoxic lymphocytes. Titration experiments show that suppressor/cytotoxic lymphocytes are more active in the regression assay that unfractionated E+ cells. When Ia+ E+ and Ia- E+ cells are separated one week after initiation of co-cultures of E+ cells and EBV-infected E- cells, both Ia+ E+ and Ia- E+ cells are active in the regression assay although regression occurs earlier in cultures receiving Ia+ E+ cells. Experiments in which NK cells are isolated using the monoclonal antibodies H25 and H366 show that NK cells do not influence the regression phenomenon in normal individuals.

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