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J Immunol Methods. 1983 Apr 29;59(2):217-20.

A simple quantitative protein A micro-immunoprecipitation method; assay of antibodies to the N and H antigens of poliovirus.

Abstract

Staphylococcus aureus (Cowan strain I) was used to absorb immune complexes from antiserum to poliovirus to which labeled N or H poliovirus antigens had been added, and the radioactivity in the pelleted organisms and in the supernatant was measured. Excellent agreement was obtained between values calculated separately from the pellet and supernatant readings, validating the use of supernatant measurements from a microtitration plate method.

PMID:
6302166
DOI:
10.1016/0022-1759(83)90033-9
[Indexed for MEDLINE]

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