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Am J Physiol. 1983 Jan;244(1):C3-10.

Nonenzymatic separation of myocardial cell nuclei from whole heart tissue.

Abstract

A simple and rapid nonenzymatic method has been developed to isolate myocardial cell nuclei from whole heart tissue. This method consists of a controlled disruption of cells followed by isopycnic gradient centrifugation. We have reviewed and compared our method to others more lengthy and laborious. By using a number of criteria, such as morphometric measurements, chemical composition, functional studies, specific nuclear protein markers, and mathematical analysis, we show that the nonenzymatic digestion method provides a most useful technique for the study of the biochemistry of the myocardial cell nucleus.

PMID:
6295177
DOI:
10.1152/ajpcell.1983.244.1.C3
[Indexed for MEDLINE]

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