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Nucleic Acids Res. 1982 Oct 11;10(19):5765-72.

pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.

Abstract

A multipurpose plasmid, pUR 250, has been constructed. It contains seven unique cloning sites (Hind III, Xba I, Sal I, Acc I, Hinc II, BamH I and EcoR I) in a small region of its lac Z gene part. Insertion of foreign DNA into the plasmid can be easily detected. Plasmid DNA isolated from a clone by a rapid method can be used to determine the DNA sequence of the insert from both ends directly without isolation of labeled fragment.

PMID:
6292850
PMCID:
PMC320928
DOI:
10.1093/nar/10.19.5765
[Indexed for MEDLINE]
Free PMC Article

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