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Gene. 1982 Feb;17(2):229-33.

Molecular cloning of the gene for dihydrofolate reductase from Lactobacillus casei.

Abstract

The Lactobacillus casei gene for dihydrofolate reductase has been cloned in Escherichia coli using the multicopy vector pBR322. A restriction map of the cloned DNA has been prepared. The cloned DNA directs the synthesis of L. casei dihydrofolate reductase in E. coli and confers trimethoprim and methotrexate resistance.

PMID:
6282715
[PubMed - indexed for MEDLINE]
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