Immunization of cattle with a variant-specific surface antigen of Trypanosoma brucei: influence of different adjuvants

Infect Immun. 1982 Apr;36(1):1-10. doi: 10.1128/iai.36.1.1-10.1982.

Abstract

Nine different adjuvants were examined for their ability to potentiate the humoral and cell-mediated immune responses of cattle to a soluble glycoprotein antigen prepared from Trypanosoma brucei. Serological responses as measured by the Farr assay were best augmented by the oil-based adjuvants and saponin. Cell-mediated immunity, as assessed by specific lymphocyte transformation in vitro, was enhanced by all oil-based adjuvants at different intervals after immunization. Results from a challenge infection of immunized cattle with the homologous clone of T. brucei and from neutralization tests indicated that protection against infection was better correlated with specific antibodies than with cell-mediated responses. From these considerations, and the absence of tissue reactions at the site of inoculation, saponin was considered more practical than the oil-based or bacterial adjuvants for the elicitation in cattle of antibodies to purified soluble antigens.

MeSH terms

  • Acetylmuramyl-Alanyl-Isoglutamine / immunology
  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Antibody Formation
  • Antigens, Surface / immunology*
  • Bordetella pertussis / immunology
  • Cattle / immunology
  • Freund's Adjuvant / pharmacology
  • Immunization / veterinary*
  • Lymphocyte Activation
  • Poly A-U / immunology
  • Propionibacterium acnes / immunology
  • Trypanosoma brucei brucei / immunology*
  • Trypanosomiasis, Bovine / immunology*
  • Trypanosomiasis, Bovine / prevention & control

Substances

  • Adjuvants, Immunologic
  • Antigens, Surface
  • Poly A-U
  • Acetylmuramyl-Alanyl-Isoglutamine
  • Freund's Adjuvant