Two new restriction endonucleases from Proteus vulgaris

Nucleic Acids Res. 1981 Sep 25;9(18):4525-36. doi: 10.1093/nar/9.18.4525.

Abstract

Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5' C A G decrease C T G 3' 3' G T C increase G A C 5' and cleave as indicated by the arrow (decrease). PvuI is an isoschizomer of XorII, RshI, and XniI. No enzyme with the specificity of PvuII has been described previously.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • DNA Restriction Enzymes / isolation & purification
  • DNA Restriction Enzymes / metabolism*
  • Deoxyribonucleases
  • Pancreas / enzymology
  • Proteus vulgaris / enzymology*
  • Substrate Specificity

Substances

  • Deoxyribonucleases
  • DNA Restriction Enzymes