Terminal strand-switching of E. coli RNA polymerase transcribing a truncated DNA fragment

Biochim Biophys Acta. 1981 Oct 27;655(3):446-8. doi: 10.1016/0005-2787(81)90056-3.

Abstract

When transcribing a restriction fragment containing the promoters and the first part of the rrnE operon of Escherichia coli, RNA polymerase holoenzyme starts exclusively on the promoters. Besides run-off transcripts, molecules longer than template-size are formed by terminal strand switch.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Restriction Enzymes
  • DNA, Bacterial / genetics*
  • DNA-Directed RNA Polymerases / metabolism*
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Microscopy, Electron
  • Molecular Weight
  • Operon
  • Transcription, Genetic*

Substances

  • DNA, Bacterial
  • DNA-Directed RNA Polymerases
  • DNA Restriction Enzymes