Abstract
A new sequence-specific endonuclease, StuI, produced by Streptomyces tubercidicus KCC S-0054, was identified and partially purified. StuI recognizes the hexanucleotide "palindromic" sequence (Formula: see text), and cleaves it at the middle, producing blunt ends.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA Restriction Enzymes / isolation & purification*
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DNA Restriction Enzymes / metabolism
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Deoxyribonucleases, Type II Site-Specific*
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Streptomyces / enzymology*
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Substrate Specificity
Substances
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DNA Restriction Enzymes
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AGGCCT-specific type II deoxyribonucleases
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Deoxyribonucleases, Type II Site-Specific