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J Hyg (Lond). 1981 Feb;86(1):79-85.

Detection and quantification of IgM, IgA, IgG1 and IgG2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay.

Abstract

A simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (FMD) virus in bovine sera. The assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. The specific antibody is reacted with FMD virus, which is then detected by an enzyme-labelled anti virus IgG.

PMID:
6257779
PMCID:
PMC2134065
DOI:
10.1017/s0022172400068765
[Indexed for MEDLINE]
Free PMC Article

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