Changes in form of elongation factor 1 during germination of wheat seeds

Eur J Biochem. 1984 Feb 15;139(1):1-4. doi: 10.1111/j.1432-1033.1984.tb07967.x.

Abstract

Two forms of EF-1 are present in the high-speed supernatant fraction from wheat embryo homogenate. In embryos from dry seeds EF-1H is 55% of the total amount of EF-1, while after 40 h of germination this form completely disappears. When germination and protein synthesis are accelerated by means of 6-benzyladenine, the rate of conversion of EF-1H is increased. On the other hand, the block of germination and of the evolution of protein synthesis by abscisic acid, block this conversion; the block of water uptake, that stops germination and causes a decrease in protein synthesis, reverses the conversion of EF-1H to EF-1L, increasing EF-1H from 15% to 40% of the total.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology
  • Adenine / analogs & derivatives
  • Adenine / pharmacology
  • Benzyl Compounds
  • Chemical Phenomena
  • Chemistry
  • Gibberellins / pharmacology
  • Kinetin
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors / metabolism*
  • Plant Proteins / biosynthesis*
  • Purines
  • Seeds / metabolism*
  • Triticum / metabolism*
  • Water

Substances

  • Benzyl Compounds
  • Gibberellins
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors
  • Plant Proteins
  • Purines
  • Water
  • Abscisic Acid
  • gibberellic acid
  • Adenine
  • benzylaminopurine
  • Kinetin