U2 region of Epstein-Barr virus DNA may encode Epstein-Barr nuclear antigen 2

Proc Natl Acad Sci U S A. 1984 Dec;81(23):7632-6. doi: 10.1073/pnas.81.23.7632.

Abstract

Sequence analysis of the U2 regions of the B95-8 and AG876 Epstein-Barr virus (EBV) isolates reveals divergence within a long open reading frame previously identified as encoding 1.5 kilobases of the 3' end of a viral RNA expressed in latently infected, growth-transformed, B-lymphocyte cell lines. Differences among EBV isolates within the U2 open reading frame are shown to correlate with differences in an EBV nuclear antigen, EBNA2. B95-8, W91, Raji, Cherry, and Lamont EBV isolates have similar U2 domains and encode similar-size EBNA2 proteins, while AG876, Jijoye, and P3HR-1 have variant or absent U2 domains and variant or absent EBNA2 proteins. The AG876 U2 open reading frame and EBNA2 protein are both shorter than those of B95-8. These data indicate that the U2 open reading frame encodes EBNA2.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Viral / genetics*
  • Base Sequence
  • Cell Line
  • DNA Restriction Enzymes
  • DNA, Recombinant / analysis
  • DNA, Viral / genetics*
  • DNA, Viral / isolation & purification
  • Epstein-Barr Virus Nuclear Antigens
  • Genes
  • Genes, Viral
  • Herpesvirus 4, Human / genetics*
  • Herpesvirus 4, Human / immunology
  • Nucleic Acid Hybridization
  • Protein Biosynthesis
  • RNA / genetics*
  • RNA, Small Nuclear
  • Virion / genetics

Substances

  • Antigens, Viral
  • DNA, Recombinant
  • DNA, Viral
  • Epstein-Barr Virus Nuclear Antigens
  • RNA, Small Nuclear
  • RNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/K03332
  • GENBANK/K03333