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Nucleic Acids Res. 1983 Jul 25;11(14):4823-33.

Structural features required for the binding of tRNATrp to avian myeloblastosis virus reverse transcriptase.

Abstract

The basis of the specific binding of tRNATrp by avian myeloblastosis virus reverse transcriptase was studied by chemical and enzymatic modification of the RNA. Binding does not depend on recognition of the tryptophan anticodon since molecules cleaved in the anticodon are stably bound by the enzyme. Modification of pseudouridine residues in the tRNA destroys binding to reverse transcriptase. These results are consistent with a model in which reverse transcriptase-tRNATrp interaction occurs not at the anticodon, but at regions in the tRNA which contain or are stabilized by pseudouridine residues.

PMID:
6192393
PMCID:
PMC326088
[Indexed for MEDLINE]
Free PMC Article

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