Mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo

Cancer Res. 1983 Apr;43(4):1790-7.

Abstract

An assay to measure endothelial cell mobilization on a gelatin substratum has been developed. Utilization of the gelatin-agarose and Boyden chamber assays established that: (a) fragments or extracts of corneas treated with several effectors of angiogenesis in vivo acquired the capacity to mobilize the capillary endothelium in vitro; (b) this mobilization was selective for the capillary endothelium; endothelium from aorta and fibroblasts from human skin or rabbit cornea were unresponsive; and (c) among the effectors of angiogenesis utilized alone; i.e., without the intermediary action of the cornea, none were able to mobilize the capillary endothelium in vitro, except for the heparin-copper complex. The data are interpreted to indicate that new formation of capillaries in vivo is the end result of a cascade of events of which heparin and copper are important components.

MeSH terms

  • Adrenal Glands / blood supply
  • Animals
  • Aorta / physiology*
  • Capillaries / physiology*
  • Cattle
  • Cell Line
  • Cell Movement
  • Clone Cells
  • Cornea / physiology*
  • Endothelium / physiology*
  • Fibroblasts / physiology
  • Gelatin
  • Neovascularization, Pathologic*
  • Sepharose

Substances

  • Gelatin
  • Sepharose