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Mol Biol Rep. 1982 Nov 30;8(4):213-6.

Synthesis of full-length cDNAs from partially purified human procollagen mRNAs.

Abstract

Partially purified human procollagen mRNAs have been copied using reverse transcriptase from avian myeloblastosis virus. Under standard conditions, using a high deoxynucleotide concentration, only incomplete cDNAs corresponding to one-fifth to one-half of the template could be synthesized. Addition of a ribonuclease inhibitor from human placenta in the reaction mixture allowed the synthesis of full-length cDNA copies from extremely long procollagen mRNA.

PMID:
6186901
[Indexed for MEDLINE]
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