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Biochem J. 1984 Jul 15;221(2):477-82.

The Ca2+-activated polyphosphoinositide phosphodiesterase of human and rabbit neutrophil membranes.

Abstract

Addition of Ca2+ to a plasma-membrane fraction derived from human or rabbit neutrophils led to the specific breakdown of polyphosphoinositides. The degradation products were identified as diacylglycerol and inositol bis- and tris-phosphate, thus demonstrating the presence of a Ca2+-activated phospholipase C. The newly generated diacylglycerol resembled the polyphosphoinositides in its fatty acid composition, and in the presence of MgATP2- it was converted into phosphatidate. These results therefore demonstrate the presence in neutrophil plasma membranes not only of polyphosphoinositide phosphodiesterase but also of diacylglycerol kinase.

PMID:
6089740
PMCID:
PMC1144062
[Indexed for MEDLINE]
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