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Stain Technol. 1976 Mar;51(2):131-4.

A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.

Abstract

This paper describes a modification of the Simons and Van Horn (1971) procedure for rendering cartilage blue, bone red, and soft tissue translucent or transparent in whole vertebrate specimens. Alcian blue and alizarin red S are used to stain cartilage and bone respectively. In our procedure formalin is used as a fixative. This is a significant modification because formalin is the common fixative for museum specimens. This clearing and staining procedure is thus readily applicable to comparative studies in anatomy, embryology and systematic zoology.

PMID:
59420
DOI:
10.3109/10520297609116684
[Indexed for MEDLINE]

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