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Nucleic Acids Res. 1978 Nov;5(11):4155-63.

Partial purification of transcriptionally active nucleosomes from trout testis cells.

Abstract

Mononucleosomes (MN1) enriched in structural non-histone proteins and transcribed DNA sequences were obtained by limited digestion of trout testis nuclei with micrococcal nuclease followed by selective solubilization in 0.1 M NaCl. These monosomes consist of the four inner histones plus stoichiometric amounts of the non-histone protein H6, of the HMG group, complexed with 140 base pairs of DNA. Hybridization experiments indicate that MN1 DNA is enriched in sequences complementary to cytoplasmic polyadenylated RNA.

PMID:
569293
PMCID:
PMC342740
[Indexed for MEDLINE]
Free PMC Article
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