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Biochem J. 1968 Mar;107(1):1-6.

The kinetics of the reduction of isocyanides, acetylenes and the cyanide ion by nitrogenase preparation from Azotobacter chroococcum and the effects of inhibitors.

Abstract

1. Nitrogen-fixing preparations from Azotobacter chroococcum reduced substrates with the following K(m) values: methyl isocyanide, 1.8x10(-4)m; ethyl isocyanide, 2.5x10(-2)m; cyanide ion, 1.4x10(-3)m; acetylene, 1.2x10(-4)m. 2. Nitrogen, carbon monoxide or hydrogen competitively inhibited isocyanide reduction with the following K(i) values: hydrogen, 1.3x10(-3)m; carbon monoxide, 6.8x10(-6)m; nitrogen, 4.3x10(-4)m. 3. Living nitrogen-fixing bacteria, and isolated clover nodules, formed methane from methyl isocyanide. 4. These results are discussed in relation to other work and possible mechanisms of nitrogen fixation.

PMID:
5642620
PMCID:
PMC1198603
DOI:
10.1042/bj1070001
[Indexed for MEDLINE]
Free PMC Article

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