Format

Send to

Choose Destination
Stain Technol. 1975 Sep;50(5):319-29.

The use of an optical brightener in the study of plant structure.

Abstract

An optical brightener Calcoflour White M2R New has been used to stain cell walls of higher plants. It can be used either as a vital stain for intact plants or for hand sections and plastic-embedded thin sections. Walls are brilliantly fluorescent while most cytoplasmic components are normally unstained. The brightener binds strongly to cellulose, carboxylated polysaccharides, and callose. Staining for 20 sec to 2 min in a 0.01% solution of the brightener is preferred for most purposes.

PMID:
54956
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center