Polymorphism of the migration of double-stranded RNA genome segments of reovirus isolates from humans, cattle, and mice

J Virol. 1979 Jul;31(1):104-11. doi: 10.1128/JVI.31.1.104-111.1979.

Abstract

A series of 94 isolates of reovirus from humans, cattle, and mice, showed extensive variability in the patterns of migration of the ten double-stranded RNA genome segments. This variation was found in all three serotypes, and involved all ten genome segments, including the segment responsible for serological specificity. Although a single pattern was present among several samples isolated from individuals and collected at a single time and place, there were often multiple genetic variants of a single serotype present in a population. Samples isolated from widely different geographic origins or different mammalian hosts showed different patterns; samples from a single species from the same area over a period of time showed more limited variations. Among most isolates, the migration of the slowest S segment, the segment that encodes the hemagglutinin and is responsible for serological specificity in laboratory strains, was similar to reference strains for type 1 and type 3 isolates. However, the type 2 isolates showed considerable variation in this segment.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anal Canal / microbiology
  • Animals
  • Cattle / microbiology
  • Electrophoresis, Polyacrylamide Gel
  • Feces / microbiology
  • Genes, Viral*
  • Humans
  • Mammalian orthoreovirus 3 / analysis
  • Mice / microbiology
  • Pharynx / microbiology
  • Polymorphism, Genetic
  • RNA, Double-Stranded / analysis*
  • RNA, Viral / analysis*
  • Reoviridae / analysis*

Substances

  • RNA, Double-Stranded
  • RNA, Viral