Microchromatographic quantitation of fetal hemoglobin in patients with sickle cell disease

Hemoglobin. 1979;3(5):341-51. doi: 10.3109/03630267908997539.

Abstract

A microchromatographic procedure (Isolab Fast Hb Test System) which was developed for the quantitation of Hb AI (10) has been found useful for the quantitation of Hb F in samples that contain Hb S and/or Hb C but no Hb A (% Fmicro). This method has been evaluated through analyses of known mixtures of Hb F and Hb S. The Hb Fmicro levels in patients with sickle cell anemia and related conditions were compared with results obtained by alkali denaturation (% FAD) and conventional DEAE-cellulose chromatography (% FDE). This microchromatographic technique is a fast, simple, and sensitive method for Hb F quantitation in patients with Sickle cell disease.

MeSH terms

  • Adult
  • Anemia, Sickle Cell / blood*
  • Chromatography / methods
  • Chromatography, DEAE-Cellulose
  • Fetal Hemoglobin*
  • Hemoglobin, Sickle
  • Hemolysis
  • Humans
  • Infant, Newborn
  • Time Factors

Substances

  • Hemoglobin, Sickle
  • Fetal Hemoglobin