Utility of sodium hypochlorite for ultrastructure study of bacterial spore integuments

J Bacteriol. 1966 Dec;92(6):1772-8. doi: 10.1128/jb.92.6.1772-1778.1966.

Abstract

Rode, L. J. (The University of Texas, Austin), and M. Glenn Williams. Utility of sodium hypochlorite for ultrastructure study of bacterial spore integuments. J. Bacteriol. 92:1772-1778. 1966.-Spores of Bacillus megaterium are partially dissolved by sodium hypochlorite. Spore integuments become visible during the dissolution, and ultrastructural features may be detected. Three distinct integument types are described for B. megaterium QM B1551 with the use of this technique. Since a variety of microbial cells are affected by sodium hypochlorite, its use may be applicable to ultrastructure study of cells other than bacterial spores.

MeSH terms

  • Bacillus megaterium / cytology*
  • Colorimetry
  • Hydrogen-Ion Concentration
  • Microscopy, Electron
  • Sodium Hypochlorite / pharmacology*
  • Spores

Substances

  • Sodium Hypochlorite