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Clin Exp Immunol. 1976 Jan;23(1):166-74.

A simple method for the cryopreservation of lymphocytes. Retention of specific immune effector functions by frozen-stored cells.

Abstract

A simple, quick and inexpensive method for cryostorage of lymphocytes is discribed. When injected into appropriate normal recipients, frozen-stored rat and sheep lymphocytes caused GVH and NLT reactions respectively. Sheep lymphocytes remained viable after several months storage and functioned satisfactorily as 51Cr-labelled target cells in assays for cytotoxic antibodies. Lymphocytes, including immunoblasts, drained from sheep efferent lymphatics during immune responses to injected murine P815 cells or allogeneic lymphocytes survived freezing; when thawed the cells retained specific immune cytotoxic effector functions including the ability to secrete complement-dependent and leucocyte-dependent antibodies.

PMID:
4251
PMCID:
PMC1538356
[Indexed for MEDLINE]
Free PMC Article

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