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Anal Biochem. 1985 Sep;149(2):516-20.

A convenient S-2-aminoethylation of cysteinyl residues in reduced proteins.

Abstract

The cysteinyl residues in proteins had been S-2-aminoethylated with ethylenimine quantitatively. However, ethylenimine is now listed as a carcinogen and is no longer commercially available. A method of converting cysteinyl residues to S-2-aminoethyl derivatives quantitatively using 2-bromoethylamine under mild conditions was developed here.

PMID:
4073506
[Indexed for MEDLINE]

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