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Virology. 1985 Jul 30;144(2):448-56.

Switch-on of transcriptase function in reovirus: analysis of polypeptide changes using 2-D gels.

Abstract

Two-dimensional gel electrophoresis (IEF and SDS-PAGE) was used to examine virion polypeptide changes associated with switch-on of transcriptase function in reovirus. Results reveal that switch-on is correlated with altered electrophoretic behavior of a specific minor polypeptide (delta 1) which is present in intermediate subviral particles. A second finding is that each of the molecular weight classes of viral polypeptides exists as a series of subspecies with different isoelectric points. This suggests that extensive posttranslational modification of progeny viral polypeptides occurs during particle morphogenesis. These findings have important theoretical and practical implications.

PMID:
4060593
DOI:
10.1016/0042-6822(85)90285-5
[Indexed for MEDLINE]

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