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J Virol Methods. 1985 Jul;11(3):225-30.

Rapid purification of HBsAg from human plasma.


A method is described for the rapid isolation of purified HBsAg from infected donor plasma. This method uses polyethylene glycol precipitation and a single isopycnic ultracentrifugation step in which the gradient is formed in situ. It works equally well using a small swingout rotor or a zonal rotor.

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