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J Antimicrob Chemother. 1986 Jan;17(1):37-44.

Accumulation of gentamicin by Staphylococcus aureus: the role of the transmembrane electrical potential.

Abstract

Accumulation of gentamicin by gentamicin-susceptible Staphylococcus aureus was examined by using the ionophorous antibiotic, valinomycin and the protontranslocating ATPase inhibitor N,N'-dicyclohexylcarbodiimide. The effects of these inhibitors on the transmembrane electrical potential (delta psi) were determined by measuring the equilibrium distribution of the tetraphenylphosphonium ion. The results indicate a direct correlation between delta psi and the extent of gentamicin uptake. However, under conditions where a significant delta psi existed across the plasma membrane, uptake of gentamicin was negligible. A threshold delta psi may thus be required to initiate gentamicin uptake. The proposed threshold delta psi appears to vary depending upon the external concentration of gentamicin.

PMID:
3949638
DOI:
10.1093/jac/17.1.37
[Indexed for MEDLINE]

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