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Anal Biochem. 1985 Aug 15;149(1):166-8.

Rapid chromatographic purification of apolipoproteins A-I and A-II from human plasma.

Abstract

Rapid, large-scale isolation of human apolipoproteins A-I and A-II has been accomplished using two chromatographic procedures. The apolipoproteins adsorbed from plasma onto a column of phenyl-Sepharose are eluted with increasing propylene glycol concentrations. Apolipoproteins A-I and A-II can be resolved by elution with a linear 0 to 80% propylene glycol gradient. Homogeneous preparations of apo A-I and A-II are obtained following gel filtration in 3M guanidinium chloride.

PMID:
3935001
DOI:
10.1016/0003-2697(85)90490-7
[Indexed for MEDLINE]

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