Format

Send to

Choose Destination
Cell. 1985 Jul;41(3):763-9.

Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins.

Abstract

In these experiments we demonstrate that purified RAS proteins, whether derived from the yeast RAS1 or RAS2 or the human H-ras genes, activate yeast adenylate cyclase in the presence of guanine nucleotides. These results confirm the prediction of earlier genetic and biochemical data and for the first time provide a complete biochemical assay for RAS protein function. Furthermore, we observe a biochemical difference between the RAS2 and RAS2val19 proteins in their ability to activate adenylate cyclase after preincubation with GTP.

PMID:
3891097
DOI:
10.1016/s0092-8674(85)80057-x
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center