Abstract
An intracellular monocyte derived protein possessing interleukin 1 (IL-1) activity has been compared with the secreted from of IL-1. Our results indicated that this intracellular IL-1 had an apparent size greater than that of extracellular IL-1. Data are presented to show that a rabbit heterotypic antiserum prepared against epitopes on the secreted form of human IL-1 blocked the biologic activity of intracellular IL-1 in a dose dependent manner. Intracellular IL-1 had a mass of 30-40 kDa as determined by gel filtration using sephacryl-200 gel.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cytoplasm / physiology
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Extracellular Space / physiology
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Growth Substances / isolation & purification
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Humans
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Interleukin-1 / isolation & purification
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Interleukin-1 / physiology*
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Interleukin-4
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Lymphokines / isolation & purification
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Macrophages / physiology
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Molecular Weight
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Monocytes / physiology
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Monokines
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Protein Precursors / physiology*
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Proteins / metabolism*
Substances
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Growth Substances
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Interleukin-1
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Lymphokines
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Monokines
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Protein Precursors
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Proteins
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Interleukin-4