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J Immunol Methods. 1985 Nov 7;83(2):209-15.

Detection of eosinophils using an eosinophil peroxidase assay. Its use as an assay for eosinophil differentiation factors.


A colorimetric assay for peroxidase has been applied to the detection of eosinophils in bone marrow cultures and tissue cell suspensions. The substrate solution consists of 0.1 mM o-phenylenediamine in 0.05 M Tris-HCl buffer pH 8.0 containing 0.1% Triton and 1 mM hydrogen peroxide. The method is shown to be an easy and reproducible method of detecting eosinophils, with insignificant interference from neutrophils.

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