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Gene. 1979 May;6(1):23-8.

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Abstract

Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment. With 24-h-old competent cells we obtained routinely 2 . 10(7) transformants per microgram of pBR322 DNA, and transformed over 20% of viable cells.

PMID:
383576
DOI:
10.1016/0378-1119(79)90082-9
[Indexed for MEDLINE]

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