Chloroflexus sp. MS-CIW-1 was isolated from a phototrophic mat in Mushroom Spring, an alkaline hot spring in Yellowstone National Park, WY, USA. We report the draft genome of 4.8 Mb consisting of 6 contigs with 3755 protein-coding genes and a GC content of 54.45%.
Keywords: Chloroflexus; Mushroom Spring; anoxygenic phototroph; chloroflexota; genomes; hot spring; phototrophic mat; phototrophs.