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J Immunol Methods. 1979;28(1-2):187-92.

ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes.


A method is described which permits the adaption of ELISA techniques for measurement of antibody against bacterial polysaccharides. First, the polysaccharides antigen is covalently bound to poly-L-lysine, using cyanuric chloride as the coupling agent. The poly-L-lysine then adsorbs to the walls of plastic tubes, thus immobilizing the polysaccharide coupled to the poly-L-lysine. The method is simple, rapid, and utilizes small amounts of polysaccharide antigen.

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