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Mycopathologia. 1979 Mar 30;67(1):39-43.

Lipid synthesis during reinitiation of growth from stationary phase cultures of Candida albicans.


Lipid synthesis has been studied in the dimorphic fungus Candida albicans. 14C-acetate incorporation into lipid material was used to measure new lipid synthesis in two cultures in which either yeast or mycelial growth was initiated from stationary phase yeast cells. When resuspended in fresh medium at 37 degrees C, cells resume budding growth. When resuspended at the appropriate temperature, both yeast and germ tube cultures immediately incorporated 14C-acetate into lipid material. The labeled lipid was more or less evenly divided between neutral and phospholipid. Phosphatidyl choline was the major phospholipid fraction and along with phosphatidyl ethanolamine accounted for 60--65% of the total phospholipid. Lipid synthesis during growth initiation of either morphology showed a similar pattern, with no significant differences observed in neutral or phospholipid or phospholipid components between yeast and mycelial forms.

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