Preparation, characterization, and in vitro cytotoxicity activity of allyl-isothiocyanate-embedded polymeric nanoparticles for potential breast cancer targeting

Background: Allyl isothiocyanate (AITC) is an excellent active phytoconstituent recently revealed for cancer treatment. The strategic prominence of this study was to synthesize and characterize AITC-embedded tripolyphosphate-modified chitosan nanoparticles (AITC@CS-TPP-NPs) by ionic gelation.

Method: Chitosan is recycled as a polymer to fabricate AITC@CS-TPP-NPs; the fabricated nanoparticles (NPs) are then characterized using FT-IR spectroscopy, DSC, XRD, zeta potential, size analysis, SEM, EDX, entrapment efficiency, in vitro drug release study, and in vitro cytotoxicity activity against MCF-7 to explore the effectiveness and strength.

Results: As a result, developed AITC@CS-TPP-NPs demonstrates good stability with a zeta potential of 35.83 mV and 90.14% of drug release. The anticancer potential of AITC@CS-TPP-NPs shows the improved cytotoxicity activity of AITC due to the surface modification of CS using TPP. Hence, the cytotoxicity of AITC@CS-TPP-NPs was tested in vitro against a human breast cancer cell line (MCF-7) and found to be considerable.

Conclusion: The AITC@CS-TPP-NPs were effectively synthesized and have significant benefits, including being easy to prepare, stable, and affordable with wide use in human breast cancer against cell line (MCF-7).