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J Immunol Methods. 1979;26(3):283-90.

A sensitive hemolytic assay for mouse C3.


We have developed a sensitive hemolytic assay for mouse C3, utilizing readily available guinea pig complement components and standard techniques, which allows C3 to be detected in mouse serum diluted many thousand-fold. Mouse serum also contains a potent, relatively heat-stable C3-inactivator system, which is largely blocked by Suramin.

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