Format

Send to

Choose Destination
Stain Technol. 1986 Jul;61(4):231-4.

Modified clearing method to show sieve tubes in minor veins of leaves.

Abstract

Leaf samples of Glycine max and numerous other dicotyledonous species were cleared by a common, well established procedure modified by using more concentrated (10% w/v) aqueous NaOH, and by leaving samples in NaOH for 2-4 weeks and in chloral hydrate for 3 days, all at room temperature. A single dye, chlorazol black E (1 g/100 ml absolute ethanol), is used to stain for 3-6 min. Samples are mounted with the lower epidermis upward. Sieve tubes in favorable material can be seen in minor veins and vein endings.

PMID:
3750353
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center