Abstract
Bovine chromaffin granules and large dense-cored vesicles of bovine splenic nerve were compared by one- and two-dimensional immunoblotting. Both types of vesicles contain chromogranin A, B and C. However, the proteolytic processing of these chromogranins within these vesicles is apparently different. Chromogranin B in chromaffin granules is processed by more than 80% whereas in nerve vesicles only 15% has been broken down to smaller proteins. In addition both types of vesicles contain dopamine beta-hydroxylase and cytochrome b-561.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens / analysis
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Cattle
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Chromaffin Granules / analysis*
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Chromaffin System / analysis*
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Chromogranins / analysis*
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Chromogranins / immunology
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Cytochrome b Group / analysis*
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Cytochrome b Group / immunology
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Dopamine beta-Hydroxylase / analysis*
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Dopamine beta-Hydroxylase / immunology
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Nerve Tissue Proteins / analysis*
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Sympathetic Nervous System / analysis*
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Sympathetic Nervous System / immunology
Substances
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Antigens
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Chromogranins
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Cytochrome b Group
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Nerve Tissue Proteins
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cytochrome b561
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Dopamine beta-Hydroxylase