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Diabetes Res. 1986 Feb;3(2):77-82.

Influence of glucose on collagen and protein production in cultured human skin fibroblasts from diabetic and non-diabetic subjects.


The effect of glucose on extracellular production of collagen and protein was studied in cultured human skin fibroblasts. Fibroblasts from 8 non-diabetics, 8 type 1 and 12 type 2 diabetic subjects were cultured. Incorporation of tritium labeled proline into medium collagen and protein was studied at glucose concentration 1-7 mg/ml (5.6-38.9 mM). A 75% increase in collagen production was noted by cells from type 1 diabetics at glucose concentration of 5 mg/ml, as compared to 1 mg/ml. A considerably lower although significant increase was also noted at this level by cells from non-diabetics. Glucose had no effect on collagen and minimal on protein production by cells from type 2 diabetic subjects. The results indicate an effect of glucose on extracellular matrix formation in diabetes mellitus which might be relevant for diabetic angiopathy.

[Indexed for MEDLINE]

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